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C1V1 = C2V2 Dilution Calculator

Solve the dilution equation for the missing concentration or volume. Enter the three known values and choose what you want to calculate.

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Calculator

Enter the three known values, then choose what to solve for. Volume units (e.g., mL)

Educational use only. Always interpret results in clinical context and verify with local policies.

How C1V1 = C2V2 Works

The C1V1=C2V2 equation represents conservation of solute: the amount of drug/solute before dilution equals the amount after dilution. When you add diluent, you increase the final volume (V2), so the final concentration (C2) decreases.

Key safety point: use final total volume (V2) — not the volume of diluent added. Diluent volume is calculated separately as V2 − V1.

Formula

C1 × V1 = C2 × V2

Worked Examples

Example 1: You have 10 mg/mL stock. You want 1 mg/mL in a final volume of 50 mL. What volume (V1) of stock do you need?

10 × V1 = 1 × 50
V1 = 50 ÷ 10 = 5 mL
Diluent = 50 − 5 = 45 mL
Answer: 5 mL stock + 45 mL diluent = 50 mL at 1 mg/mL

Example 2: You take 20 mL of 5 mg/mL solution. What concentration (C2) do you get if you make it up to 100 mL?

5 × 20 = C2 × 100
C2 = (5 × 20) ÷ 100 = 1 mg/mL
Answer: 1 mg/mL

When This Is Used

  • •Preparing diluted solutions from concentrated stock
  • •Adjusting medication concentrations for paediatric dosing
  • •Pharmacy compounding and aseptic preparation
  • •Double-checking dilution and concentration arithmetic

Clinical safety note: Always confirm concentrations, units, and local protocols. Use independent double-checks for high-risk medications and small-volume dilutions.

Related Calculators

C1 → C2 Final Volume

Calculate V1 from C1/C2/V2

Desired Concentration

Dose to final volume

All Dilution Calculators

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Frequently Asked Questions

Clinical reminder: Always follow local protocols and consult medication information sheets. These examples are for calculation practice only.

References & Further Reading

This calculator is based on established dilution and pharmaceutical standards from authoritative sources across healthcare disciplines.

International Standards

SI Prefixes (milli-, micro-, etc.)PDF

BIPM (Bureau International des Poids et Mesures)

Authoritative reference for metric prefixes used in unit conversions.

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Australia Resources

Drug Calculations PDF

Flinders University – Student Learning Support Service

Shows the standard “volume required” method and worked examples used in healthcare training.

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Medication Calculations PDF

University of Southern Queensland (USQ)

Provides formula-based approaches consistent with dilution and dose-to-volume calculations.

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Finding the Volume Required Web

RMIT Learning Lab (Nursing)

Explains stock strength/stock volume concepts that underpin dilution calculations.

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United Kingdom Resources

Intravenous Drug and Fluid Administration Training PDF

Gloucestershire Hospitals NHS Foundation Trust

Includes practical calculation methods used in IV preparation and dilution scenarios.

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United States Resources

Medication Calculations PDF

SUNY Upstate Medical University

Explains ratio/proportion style approaches relevant to dilution and dose-to-volume problems.

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Pharmacy Calculations Web

NCBI Bookshelf (StatPearls Publishing)

Covers solution/mixture calculation structures consistent with C1V1=C2V2 style dilution methods.

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Tip: If your answer looks wrong, it’s almost always a unit mismatch (e.g., mg/mL vs mg/L) or using diluent volume instead of final total volume (V2).

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