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Serial 2-Step Dilution

Calculate stock → intermediate → final volumes using C1V1=C2V2 at each step.

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Calculator

Educational use only. Always verify against local policy and product information.

How it works

Step 1: Use the stock concentration to prepare an intermediate solution (concentration + volume).

Step 2: Dilute the intermediate solution to your final concentration and final volume.

Formula

Step 1: Cstock × Vstock = Cinter × Vinter
Step 2: Cinter × Vinter-used = Cfinal × Vfinal

Clinical safety note: Always verify dilution steps against product information and local policy. Label intermediate solutions clearly (concentration, date/time, diluent).

Related calculators

C1V1 = C2V2 Basic

Multi-step Serial Dilution

Reconstitute to Final Bag

Frequently asked questions

Clinical reminder: Always follow local protocols and consult medication information sheets. These examples are for calculation practice only.

References & sources

Institute for Safe Medication Practices (ISMP)

Medication safety guidance and error prevention

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ACSQHC Medication Safety Standard (NSQHS)

Australian medication safety standard

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